Thermo Fisher Scientific Cytokeratin 6 Monoclonal Antibody (LL020), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

ELISA (ELISA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

LL020

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573034

Product Specific Information

Description: The monoclonal antibody LL020 recognizes human Cytokeratin 6. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. Cytokeratin 6 is a type II (basic) keratin that associates with Cytokeratin 16 and Cytokeratin 17. Cytokeratin 6 is expressed in the epidermis, outer root sheath of hair follicles, oral mucosa, suprabasal cells of stratified epithelia, and glandular epithelia. Gene mutations result in inherited skin and hair disorders. Some reports suggest that LL020 may have some crossreactivity to Cytokeratin 5.

Applications Reported: This LL020 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and ELISA.

Applications Tested: This LL020 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.