Thermo Fisher Scientific Phospho-RNA pol II CTD (Ser2) Monoclonal Antibody (3E10), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1 - 2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1.25 µg/ml

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3E10

Immunogen

Ovalbumin-conjugated peptide corresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser2 (YS(p)PTSPSYSPTSPSC) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RNA pol II CTD,uniProtId:P24928-1,ncbiNodeId:9606,antigenRange:2,antigenLength:1970,antigenImageFileName:14-9802-82_RNA_pol_II_CTD_P24928-1_Rat.svg,antigenImageFileNamePDP:14-9802-82_RNA_pol_II_CTD_P24928-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784639

Product Specific Information

Description: This 3E10 monoclonal antibody recognizes a specific phosphorylated form of RNA polymerase II subunit B1 (RPB1). It detects phosphorylated serine at amino acid position 2 (Ser2) within the C-terminal heptapeptide (YSPTSPS) repeats of RPB1. Modifications within the C-terminal domain (CTD) of RPB1 recruit RNA processing factors that govern the transcription cycle; phosphorylation at Ser2 is associated with transcriptional elongation. The 3E10 immunogen was an ovalbumin-conjugated peptide corresponding to human RPB1 CTD phosphorylated at Ser2 (YS(p)PTSPSYSPTSPSC).

Applications Reported: This 3E10 antibody has been reported for use in western blot and immunocytochemistry.

Applications Tested: This 3E10 antibody has been tested by western blot of human, mouse, and rat cell lysates and immunocytochemistry of human cell lines. This may be used at less than or equal to 2.0 µg/mL for western blot and less than or equal to 1.25 µg/mL for immunocytochemistry.

Target Information

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.