Thermo Fisher Scientific Phospho-RNA pol II CTD (Ser2) Monoclonal Antibody (3E10), eBioscience

Applications

Product Specifications

Product Specific Information

This 3E10 monoclonal antibody recognizes a specific phosphorylated form of RNA polymerase II subunit B1 (RPB1). It detects phosphorylated serine at amino acid position 2 (Ser2) within the C-terminal heptapeptide (YSPTSPS) repeats of RPB1.
Modifications within the C-terminal domain (CTD) of RPB1 recruit RNA processing factors that govern the transcription cycle; phosphorylation at Ser2 is associated with transcriptional elongation.
The 3E10 immunogen was an ovalbumin-conjugated peptide corresponding to human RPB1 CTD phosphorylated at Ser2 (YS(p)PTSPSYSPTSPSC).

Applications Reported: Western blot and immunocytochemistry.
Applications Tested: Tested by western blot of human, mouse, and rat cell lysates and immunocytochemistry of human cell lines. Recommended ≤2.0 µg/mL for WB and ≤1.25 µg/mL for ICC.

Target Information

RNA polymerase II is responsible for the synthesis of mRNA and many non-coding RNAs.
POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II.
The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS.
Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle.
Recruitment of RNA processing factors to the elongation complex is coordinated by phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.