Thermo Fisher Scientific Dynein Monoclonal Antibody (74.1), eBioscience
74 kDa 세포질 다이닌 중간사슬을 인식하는 단클론 항체로, Western blot, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능. 인간, 생쥐, 초파리 등 여러 종에 반응하며, 고순도(>90%)로 정제된 연구용 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | Assay-Dependent | - |
| ELISA | Assay-Dependent | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Fruit fly, Human, Mouse, Sheep, Pig, Rat, Xenopus |
| Published Species | Fruit fly, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 74.1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% Sodium Azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573019 |
Product Specific Information
The monoclonal antibody 74.1 recognizes all known forms of the 74 kDa intermediate chain subunit of mammalian cytoplasmic dynein.
Cytoplasmic dynein is a microtubule-stimulated ATPase responsible for movement of membrane-bound organelles toward the minus end of microtubules, commonly found at the centrosome. It is involved in the transport of lysosomes, endosomes, phagosomes, peroxisomes, mitochondria, and ER-derived vesicles to the Golgi apparatus.
During mitosis, dynein contributes to spindle positioning and chromosome movement via kinetochore localization.
This antibody recognizes dynein intermediate chain from human, mouse, rat, bovine, sheep, Xenopus, porcine, and Drosophila.
- Tested by immunocytochemistry (methanol-fixed, permeabilized cells) ≤1 µg/mL
- Tested by western blot (reduced and non-reduced HeLa lysate) ≤5 µg/mL
- Recommended to titrate for optimal assay performance
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Cytoplasmic dynein is a minus end-directed microtubule-based motor protein responsible for retrograde transport of membranous organelles from synapse to cell body.
The dynein complex includes two ~530 kDa heavy chains and intermediate chains of ~74 kDa and ~53–59 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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