Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (sl6arg), eBioscience
Human Arginase-1 인식 단일클론 항체로, WB, IHC, ICC 등 다양한 응용에 적합. Affinity chromatography로 정제된 고순도 항체(>90%)이며, PBS buffer에 0.09% sodium azide 포함. 간, 중성구, MDSC 등에서 Arg1 발현 연구에 활용 가능.
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Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (sl6arg), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–20 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | sl6arg |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865453 |
Available Conjugated Formats:
- Alexa Fluor 488
- Alexa Fluor 514
- Alexa Fluor 700
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- eFluor 660
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that converts L-arginine to urea and L-ornithine, aiding nitrogen elimination. Polyamines derived from L-ornithine are crucial for cell proliferation and detoxification from protein degradation.
Expression:
Found in liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells. In humans, macrophage expression is undetermined, whereas in mice, M2 macrophage expression is well established. Arginase-1 is commonly expressed in hepatocellular carcinomas and may appear in myeloid populations within breast cancer tumors.
Applications Reported:
Suitable for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded tissues), microscopy, and immunocytochemistry.
Applications Tested:
- IHC (P): ≤20 µg/mL (tested on human tissues, compatible with low/high pH antigen retrieval)
- ICC: ≤20 µg/mL (tested on fixed and permeabilized human cells)
Careful titration is recommended for optimal assay performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filter
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme catalyzing the conversion of L-arginine to urea and L-ornithine, the final step in the urea cycle. Resulting polyamines support cell proliferation and detoxification. By degrading arginine, Arg1 limits substrate availability for NO synthase, reducing nitric oxide production.
Expression Overview:
- Human and mouse: liver, neutrophils, MDSC, neural stem cells
- Human: expressed in blood neutrophils, not in CCR3+ granulocytes
- Mouse: hallmark of M2a macrophages
- Tumor association: myeloid cells infiltrating tumors, hepatocellular carcinomas
- Genetic defect: Argininemia (autosomal recessive disorder causing hyperammonemia)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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