Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (sl6arg), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5-20 µg/mL

-

Immunocytochemistry (ICC/IF)

20 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

sl6arg

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 514
• Alexa Fluor 700
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• eFluor 660
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865453

Product Specific Information

Description: The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine which further converts to polyamine. These polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. Expression is found in the liver, neutrophils, as well as myeloid derived suppressor cells (MDSC) and neural stem cells. Expression in macrophages has not been determined in humans, while in mice expression in M2 macrophages is well established. Arginase-1 may be expressed in the myeloid population in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas.

Applications Reported: This sl6arg antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This sl6arg antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval and can be used at less than or equal to 20 µg/mL. This sl6arg antibody has also been tested by immunocytochemistry of fixed and permeabilized human cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.