Thermo Fisher Scientific B7-H3 (CD276) Chimeric Recombinant Rabbit Monoclonal Antibody (MJ18)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Specificity:
This antibody binds to mouse B7-H3 and does not react with other B7-family molecules.

Preparation:
This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.

Target Information

CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells, and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis, and colon.

In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.