Thermo Fisher Scientific CD275 (B7-H2) Chimeric Recombinant Rabbit Monoclonal Antibody (HK5.3)
Thermo Fisher Scientific의 CD275 (B7-H2) 키메라 재조합 토끼 단클론 항체(HK5.3)는 마우스 ICOSL을 특이적으로 인식하며, ICC, Flow Cytometry, ELISA 등 다양한 응용에 적합합니다. 높은 순도(Protein A 정제)와 안정적 액상 형태로 제공되어 연구용으로 최적화되어 있습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 0.1 ng/mL |
| Inhibition Assays (IA) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HK5.3 |
| Immunogen | This antibody was raised by immunizing SD rat with mouse B7h-transfected L cells and fusing immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074541 |
Product Specific Information
Specificity: This antibody specifically binds to mouse ICOSL and competes with binding of ICOS.
Target Information
Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells, and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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