Thermo Fisher Scientific CD273 (B7-DC) Chimeric Recombinant Rabbit Monoclonal Antibody (TY25)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Specificity: This antibody binds to mouse PD-L2 and competes with PD-1 binding. It does not cross-react with PD-L1.

Target Information

Programmed death-ligand 2 (PD-L2), or B7-DC, is a member of the B7 ligand family within the immunoglobulin superfamily that, along with programmed death-ligand 1 (PD-L1), acts as a ligand for programmed cell death protein 1 (PD-1). Though expressed primarily in dendritic cells, PD-L2 expression can be induced on a wide variety of immune and non-immune cells depending on the microenvironment.

PD-L2 expression is particularly upregulated in the presence of Th2 cytokine IL-4, as well as Th1 cytokines TNF-alpha and IFN-gamma to a lesser degree. While generally expressed at lower levels compared to PD-L1, PD-L2 demonstrates a 2 to 6 times higher relative affinity to PD-1 than PD-L1.

PD-1 and its ligands are inhibitory immune checkpoint molecules that provide negative feedback during physiological homeostasis. Ligation of PD-L2 or PD-L1 inhibits activation, proliferation, and cytokine secretion (e.g., IFN-gamma, IL-10) in T cells, dampening immune responses. Conversely, PD-L2 can also stimulate T cell proliferation and cytokine production, even in PD-1-deficient T cells, suggesting additional receptors.

Recent studies show PD-L2 also binds to repulsive guidance molecule b (RGMb), a receptor for bone morphogenetic proteins (BMPs). RGMb is expressed in the central nervous system and macrophages, with emerging roles in immunity. Interaction between PD-L2 and RGMb regulates respiratory tolerance development in the lung through BMP and/or neogenin signaling pathways.

The naturally occurring human PD-L2 monomer consists of a 201-amino-acid extracellular domain, a 21-amino-acid transmembrane domain, and a 32-amino-acid cytoplasmic domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.