Thermo Fisher Scientific Phospho-BAD (Ser112) Monoclonal Antibody (G.445.9)
Phospho-BAD (Ser112) 단백질 검출용 단일클론 항체로 Western blot, IHC, Flow cytometry에 적합. Rabbit IgG 기반으로 인간, 마우스, 랫드, 영장류 시료에 반응. 액상 형태, -20°C 보관, 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:800 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G.445.9 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer112 of mouse Bad |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 66 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10985837 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The protein encoded by BAD is a member of the BCL-2 family, which regulates programmed cell death. BAD promotes apoptosis by forming heterodimers with BCL-xL and BCL-2, reversing their anti-apoptotic effects. Its proapoptotic activity is regulated by phosphorylation, involving protein kinases such as AKT and MAP kinase, and phosphatase calcineurin. Alternative splicing of this gene results in two transcript variants encoding the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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