Thermo Fisher Scientific VPARP Monoclonal Antibody (P193-10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

1:10

-

Flow Cytometry (Flow)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

P193-10

Immunogen

VPARP aa 408-611

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

purified

Storage buffer

PBS with 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2158808

Product Specific Information

p193-10 reacts with an internal epitope (amino acids 506-510, VALGK) of the minor vault protein (p193 or VPARP), which is overexpressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles. p193-10 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.

Target Information

This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.