Thermo Fisher Scientific Nestin Polyclonal Antibody
인간 및 생쥐 Nestin 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제, 액상 형태, 안정적 보관 가능. 신경줄기세포 및 종양 연구용으로 이상적.
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Thermo Fisher Scientific Nestin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| ELISA | 1:20,000–1:80,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to amino acids 1484–1500 of human Nestin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
Nestin antibody is directed against human Nestin protein. BLAST analysis suggests cross-reactivity with Nestin protein from human and macaque (100% homology). Based on sequence homology, partial reactivity may occur with Nestin homologues from rabbit (94%), mouse (shown above), rat and hamster (88%), and bovine and dog (82%). Reactivity against other sources is not known.
Target Information
Nestin is a Class VI intermediate filament abundantly expressed in developing CNS and embryonic neuroepithelial stem cells. It serves as a marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also a superior angiogenic marker for evaluating neovascularity in tumors. Highly specific antibodies to human Nestin assist in characterizing progenitor cell differentiation, enhancing therapeutic potential of neural stem/progenitor cells for CNS disease or injury, and identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cells and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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