Thermo Fisher Scientific PPAR gamma 1 + 2 Polyclonal Antibody
다양한 종에서 반응성을 보이는 PPAR gamma 1+2 폴리클로날 항체. Western blot, ELISA, IP 등 다양한 응용에 적합. Rabbit IgG 기반으로 고순도 친화 크로마토그래피 정제. -20°C 보관 시 안정적이며 연구용으로 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| ELISA | 1:8,000–1:32,000 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Avian, Dog, Guinea pig, Hamster, Human, Mink, Mouse, Non-human primate, Pig, Rabbit, Rhesus monkey, Rodent, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to amino acids 250–300 of human PPAR gamma isoform 1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity purified antibody is directed against human PPAR gamma protein.
BLAST analysis suggests broad reactivity with PPAR gamma from multiple species including human (g1, g2), mouse (g1, g2), rat, squirrel, orangutan, duck (g1), macaque (g1–g7), boar (g1a–1d), mink, guinea pig, rabbit (g1, g3), dog, and hamster based on 100% homology for the immunogen sequence.
No reactivity is expected against other subtypes of PPAR.
Target Information
The PPAR gamma gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) to regulate transcription of various genes.
Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma.
PPAR-gamma regulates adipocyte differentiation and is implicated in diseases such as obesity, diabetes, atherosclerosis, and cancer.
Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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