Thermo Fisher Scientific GFP Polyclonal Antibody, HRP
GFP 단백질을 인식하는 HRP 결합 염소 폴리클로날 항체로, WB, IHC, ELISA, IP에 적합합니다. 고순도 친화 크로마토그래피 정제 제품이며, 4°C 단기 보관 및 -20°C 장기 보관이 가능합니다. 연구용으로 GFP 태그 단백질 검출에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:2,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from Aequorea victoria |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay Information:
Immunoelectrophoresis showed a single precipitin arc against anti-Goat Serum, anti-Peroxidase, and purified GFP (Aequorea victoria).
No cross-reactivity observed with Human, Mouse, or Rat serum proteins.
Target Information
The jellyfish Aequorea victoria produces Green Fluorescent Protein (GFP), which emits green light (peak 508 nm) after absorbing blue light (395 nm). GFP is widely used as:
- A reporter protein for gene expression in both eukaryotic and prokaryotic systems
- A fusion tag to study protein localization and dynamics
- A tool for fluorescence-based assays such as FRET and yeast two-hybrid systems
GFP is a 27 kDa monomeric protein forming its fluorescent chromophore autocatalytically. Its fluorescence is stable, species-independent, and suitable for diverse biological research applications.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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