Thermo Fisher Scientific Phospho-DNA-PK (Ser2056) Polyclonal Antibody
DNA 손상 반응 경로에서 Ser2056 인산화된 DNA-PK를 검출하는 토끼 폴리클로날 항체. Western blot, ICC/IF, ChIP assay에 적합하며, 항원 친화 크로마토그래피로 정제됨. 인간 및 생쥐 시료에 반응. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human DNA-PKcs (phospho Ser2056). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.76 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736365 |
Product Specific Information
- Positive Control: 293T, 293T (100 J/m² UVC treatment)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. It is an atypical kinase closely related to PI3 kinases and can be inhibited by PI3 kinase-targeting molecules.
DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex.
Multiple transcript variants encoding different isoforms have been identified for DNA-PK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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