Thermo Fisher Scientific MHC Class II Monoclonal Antibody (HIS19), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• APC
• Biotin
• FITC
• PE
• Request custom conjugation

Product Specific Information

Description:
The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except RT1n (including BN and MAXX strains). Rat MHC class II (I-A equivalent), RT1B, is expressed by antigen-presenting cells such as B cells, dendritic cells, and macrophages.

Applications Reported:
The HIS19 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.

Applications Tested:
The HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cells. Recommended usage is ≤0.5 µg per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be empirically determined (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells, and B cells. Before appearing on the cell surface, MHC class II molecules bind endocytosed exogenous antigens, which are then presented to T cells. The antigen-binding groove between MHC class II alpha and beta chains is open at both ends and is 15–24 amino acid residues long.

HLA and MHC antibodies are important in Immunopeptidomics, aiding in the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.