Thermo Fisher Scientific CD93 (AA4.1) Monoclonal Antibody (R139), eBioscience
인간 CD93(C1qRp)을 인식하는 R139 단클론 항체로, 세포 표면 단백질 연구에 적합합니다. Flow cytometry, IHC, WB, ELISA 등 다양한 응용에 사용 가능하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 4°C에서 보관하며, 연구용으로만 사용됩니다.
- 카탈로그번호
- 14-0939-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD93 (AA4.1) Monoclonal Antibody (R139), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 40 µg/mL | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 14 publications |
| ELISA | Assay-Dependent | - |
| Immunoprecipitation (IP) | Assay-Dependent | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | R139 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_891508 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The monoclonal antibody R139 recognizes human CD93 (C1qRp), a glycoprotein that binds to complement protein C1q, mannose binding lectin, and pulmonary surfactant protein A. CD93 plays a role in the clearance of apoptotic cells and is mainly expressed on myeloid cells, with higher levels on monocytes than on neutrophils, eosinophils, platelets, and endothelial cells. Expression on dendritic cells decreases upon maturation. CD93 also defines an early bone marrow stem cell population of hematopoietic and hepatic precursors.
The R139 antibody blocks C1q-mediated enhancement of phagocytosis and can detect soluble CD93 by ELISA when used in combination with R3 antibody. The epitope for R139 is located in the EGF domains.
Applications Reported: Flow cytometry, immunoprecipitation, immunoblotting (WB) under nonreducing conditions, ELISA, and immunohistochemistry of formalin-fixed paraffin-embedded tissue.
Applications Tested:
- Flow cytometric analysis of human peripheral blood cells (≤0.5 µg/test).
- Immunohistochemistry on formalin-fixed paraffin-embedded human tissue using low pH antigen retrieval (≤40 µg/mL).
Careful titration is recommended for optimal assay performance.
Target Information
The CD93 protein is a cell-surface glycoprotein and type I membrane protein originally identified as a myeloid cell-specific marker. It is involved in intercellular adhesion and clearance of apoptotic cells. The intracellular cytoplasmic tail interacts with moesin, linking transmembrane proteins to the cytoskeleton and contributing to cytoskeletal remodeling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.