Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (DA-7)
Cytokeratin 18 단백질을 인식하는 Mouse monoclonal antibody (Clone DA-7). 인간 시료에 반응하며, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 사용 가능. Protein A로 정제된 액상형 제품으로 4°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 1 publication |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DA-7 |
| Immunogen | Human breast carcinoma cell line PMC-42 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075167 |
Product Specific Information
This antibody reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of the intermediate filament subfamily of intracellular proteins found in epithelial tissues.
This antibody does not cross-react with porcine, mouse, rat, hamster, bovine, canine, or sheep.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the acidic subfamily of low molecular weight keratins and typically pairs with cytokeratin 8.
It is expressed in epithelial tissues such as those of the gastrointestinal, respiratory, and urogenital tracts, as well as endocrine and exocrine tissues and mesothelial cells.
Cytokeratin 18 is found mainly in non-squamous epithelia and is expressed in most adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas.
Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis.
Two transcript variants encoding the same protein have been identified.
Cytokeratin subtype expression patterns assist in differentiating epithelial malignancies and are valuable tools in tumor diagnosis, cytopathology, and flow cytometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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