Vazyme HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) R312

Product Introduction

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper), including a new generation of reverse transcriptase HiScript III Reverse Transcriptase and Buffer optimized for reverse transcription, further improving the efficiency of 1st strand synthesis. The 5 × gDNA wiper Mix in the kit can quickly remove genomic DNA contamination within 2 min, which makes the results more reliable and simplifies the process of qPCR primer design without the need of spanning an exon-exon junction. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers, which allows users to choose primers flexibly for subsequent experiments as required. The kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning and other downstream experiments, as well as highly uniform cDNA for qPCR.

Product Advantages

Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product;
Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs;
gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns

Components

Storage

Store at -30 ~ -15℃ for up to 18 months, and transport at ≤0℃.

10 × RT Mix contains high concentration of DTT, which may precipitate at low temperatures. Please restore to room temperature, shake gently and mix thoroughly, wait to dissolve the precipitation again before use.