Thermo Fisher Scientific TFAM Monoclonal Antibody (18G102B2E11)
TFAM 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체. 인간, 마우스, 랫트에 반응하며 WB, IHC, ICC/IF에 적합. Protein G로 정제된 액상 항체로, 1 mg/mL 농도. 미토콘드리아 DNA 복제와 전사 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 18G102B2E11 |
| Immunogen | Human mtTFA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | TBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11157422 |
Product Specific Information
Suggested positive control: HeLa whole cell lysate.
Target Information
Mitochondrial transcription factor A (TFAM) is a key nuclear-encoded protein that translocates to mitochondria, where it maintains and regulates mitochondrial DNA (mtDNA) replication and transcription. TFAM contains an LC3 interacting region (LIR) motif, enabling interaction with autophagy proteins like LC3, which is critical for mtDNA quality control and inflammation regulation. TFAM levels influence mitochondrial function across tissues—high expression can suppress mtDNA transcription in skeletal muscle, while in cardiac tissue, balanced TFAM-to-mtDNA ratios support oxidative phosphorylation (OXPHOS). Dysregulation of TFAM is linked to mitochondrial dysfunction, neurodegeneration, and diseases associated with mtDNA stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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