Thermo Fisher Scientific HELIOS Monoclonal Antibody (22F6), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific HELIOS Monoclonal Antibody (22F6), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test

Species Reactivity

• Dog
• Human
• Mouse

Host / Isotype

• Armenian hamster / IgG

Class

• Monoclonal

Type

• Antibody

Clone

• 22F6

Conjugate

• Brilliant Ultra Violet™ 395

Excitation / Emission Max

• 347 / 399 nm

Form

• Liquid

Concentration

• 5 µL/Test

Purification

• Affinity chromatography

Storage Buffer

• PBS, pH 7.2, with BSA

Contains

• 0.09% sodium azide

Storage Conditions

• 4°C, store in dark
• Do not freeze

Shipping Conditions

• Ambient (domestic)
• Wet ice (international)

RRID

• AB_2937091

Product Specific Information

This 22F6 monoclonal antibody reacts with human, mouse, and canine Helios.
Helios is a zinc-finger transcription factor similar to Ikaros, expressed in hematopoietic stem cells of embryos and adult mice.
Highest expression occurs in regulatory T cells, while mature B cells, dendritic cells, and myeloid cells do not express Helios.
Helios can form homodimers or heterodimers with other Ikaros family members to regulate transcription.
Eight isoforms exist, some with dominant-negative functions upregulated in lymphoid malignancies such as T-acute lymphoblastic leukemia.
22F6 has been reported to cross-react with rat Helios.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis.

Applications Tested:

• Pre-diluted and tested using mouse lymph node cells with Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
• Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume).
• Cell number range: 10⁵–10⁸ cells/test.

Fluorophore Information:

• Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm.
• For use on cytometers equipped with an ultraviolet (355 nm) laser.
• Verify instrument compatibility before use.

Staining Panel Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product #SB-4401-42)
• or Brilliant Stain Buffer™ (Product #00-4409-75)
  to minimize non-specific polymer interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license.
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Target Information

The Helios gene encodes a member of the Ikaros family of zinc-finger proteins.
These hematopoietic-specific transcription factors (Ikaros, Aiolos, Helios) regulate lymphocyte development.
Helios forms homo- or hetero-dimers with other Ikaros family members and functions primarily in early hematopoietic development.
Multiple transcript variants exist; biological validity of some remains uncertain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Alternative Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 563
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available

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