Thermo Fisher Scientific KLRG1 Monoclonal Antibody (13F12F2), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 786
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Custom conjugation available

Product Specific Information

This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitory receptor of the C-type lectin superfamily containing an ITIM domain. It is expressed on subsets of γδ T cells, NK (CD56dim), CD8+, and CD4+ T cells. KLRG1 is primarily found in effector/memory phenotype cells that are short-lived but capable of immediate effector function. Cadherin-E, -N, and -R serve as ligands for KLRG1, and their interaction inhibits cytolytic activity and proliferation. The percentage of KLRG1-positive cells can vary depending on antigen experience.

Clones 13F12F2 and 13A2 recognize a similar epitope based on cross-blocking studies.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (1.0 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is designed for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Cat. SB-4401-42) or Brilliant Stain Buffer™ (Cat. 00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in the cytoplasmic domain. These ITIMs interact with SH2 domains of phosphatases such as SHP-1. KLRG1 is an inhibitory receptor expressed on NK cells and certain T cells, involved in tumor and virus-infected cell lysis, and mediating humoral and cell-mediated immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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