Thermo Fisher Scientific KLRG1 Monoclonal Antibody (13F12F2), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 786
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Custom conjugation available upon request

Product Specific Information

This 13F12F2 monoclonal antibody reacts with human KLRG1 (Killer cell lectin-like receptor subfamily G, member 1), a type II transmembrane inhibitory receptor of the C-type lectin superfamily containing an ITIM domain.
KLRG1 is expressed on subsets of γδ T cells, NK (CD56dim), CD8+ and CD4+ T cells, primarily on effector/memory phenotype cells that are short-lived but capable of immediate effector function.
Cadherin-E, -N, and -R are ligands for KLRG1, and their interaction inhibits cytolytic activity and proliferation. Expression levels vary depending on antigen experience.

The clones 13F12F2 and 13A2 recognize a similar epitope based on cross-blocking studies.

Applications Reported

• Flow cytometric analysis

Applications Tested

• Pre-diluted and tested by flow cytometry using normal human peripheral blood cells
• Recommended usage: 5 µL (1.0 µg) per test (in 100 µL final volume)
• Suitable for 10⁵–10⁸ cells/test

Fluorochrome Information

• Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser.
• Ensure instrument compatibility before use.
• When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domains. These ITIMs interact with SH2 domains of protein phosphatases such as SHP-1.
KLRG1 acts as an inhibitory receptor expressed on NK cells and certain T cells, involved in the lysis of tumor and virus-infected cells, and mediating immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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