Thermo Fisher Scientific Phospho-PKC alpha (Thr497) Recombinant Rabbit Monoclonal Antibody (PKCaT497-F1), APC
Phospho-PKC alpha (Thr497) 인산화 부위를 인식하는 재조합 토끼 단클론 항체로, APC 형광체로 표지되어 있습니다. Flow Cytometry에 최적화되어 있으며, Human, Mouse, Rat 시료에 반응합니다. 단백질 A/G 정제, PBS-BSA 완충액에 보존됩니다.
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Applications
- Flow Cytometry (Flow): 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PKCaT497-F1 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKC alpha |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage buffer | PBS with 0.20% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897053 |
Additional Formats
- Unconjugated (MA5-37116)
- FITC (MA5-37118)
- PE (MA5-37117)
- Custom conjugation available upon request
Target Information
The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in structure, tissue distribution, localization, mode of action, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family, whose activities are Ca²⁺- and phospholipid-dependent. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. PKC alpha plays roles in cell adhesion, transformation, cell cycle checkpoint, and cell volume control, and may regulate cardiac contractility and Ca²⁺ handling in myocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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