Thermo Fisher Scientific Phospho-c-Abl (Tyr245) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-c-Abl (Tyr-245) peptide (without carrier).
On SDS-PAGE immunoblots of K-562 treated with pervanadate, the antibody detects a 145 kDa protein corresponding to c-Abl and a 210 kDa band corresponding to BCR-Abl.
In addition, this antibody detects a 145 kDa band in Jurkat cells treated with pervanadate.

Target Information

The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase involved in cell differentiation, division, adhesion, and stress response.
Activity of c-Abl is negatively regulated by its SH3 domain; deletion of this domain converts c-Abl into an oncogene.
Its DNA-binding activity is regulated by CDC2-mediated phosphorylation, suggesting a role in the cell cycle.
In chronic myelogenous leukemia and some acute lymphoblastic leukemias, c-Abl undergoes a (9;22) chromosomal translocation producing the Philadelphia chromosome, resulting in the BCR-Abl fusion protein.
c-Abl was first identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV).
It may regulate DNA repair by activating proapoptotic pathways when DNA damage is irreparable.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.