Thermo Fisher Scientific Phospho-c-Abl (Tyr412) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:50

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-c-Abl (Tyr-245) peptide before affinity purification using phospho-c-Abl (Tyr-412) peptide.
On SDS-PAGE immunoblots of K-562 treated with pervanadate, the antibody detects a 145 kDa protein corresponding to c-Abl and a 210 kDa band corresponding to BCR-Abl.

Target Information

The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase involved in cell differentiation, division, adhesion, and stress response.
Activity of c-Abl protein is negatively regulated by its SH3 domain; deletion of this domain converts c-Abl into an oncogene.
The DNA-binding activity of ABL1 is regulated by CDC2-mediated phosphorylation, indicating a role in the cell cycle.
In chronic myelogenous leukemia (CML) and some acute lymphoblastic leukemias (ALL), a (9;22) chromosomal translocation forms the Philadelphia chromosome, resulting in the BCR-Abl fusion protein.
c-Abl also participates in DNA repair and can activate proapoptotic pathways when DNA damage is irreparable.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.