Thermo Fisher Scientific Phospho-alpha Actinin 4 (Tyr4) Polyclonal Antibody
Phospho-alpha Actinin 4 (Tyr4) 특이적 폴리클로날 항체로, 인간·생쥐·랫드 시료에 반응. Western blot에서 100 kDa 단백질 검출 가능. 항원 친화 크로마토그래피로 정제된 고순도 제품. 연구용으로만 사용.
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Applications
- Western Blot (WB)
Tested Dilution: 1:1,000
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-alpha-actinin 4 (Tyr-4) synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminus of human alpha-actinin 4. This sequence is well conserved in rat and mouse alpha-actinin 4, but is not conserved in other alpha-actinins. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942872 |
Product Specific Information
The antibody detects a 100 kDa* protein corresponding to the molecular mass of alpha-actinin 4 on SDS-PAGE immunoblots of human A431 cells treated with pervanadate, but is not observed after alkaline phosphatase treatment.
Target Information
Alpha actinins belong to the spectrin gene superfamily representing a diverse group of cytoskeletal proteins, including alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types.
- In nonmuscle cells: localized along microfilament bundles and adherens-type junctions, binding actin to the membrane.
- In muscle isoforms (skeletal, cardiac, smooth): localized to the Z-disc and dense bodies, anchoring myofibrillar actin filaments.
This gene encodes a nonmuscle alpha actinin isoform concentrated in the cytoplasm and thought to be involved in metastatic processes. Mutations have been associated with focal and segmental glomerulosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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