Thermo Fisher Scientific Phospho-Actin (Tyr53) Polyclonal Antibody
Phospho-Actin (Tyr53) 폴리클로날 항체로 인간, 마우스, 랫트 시료에서 인산화 액틴 검출에 적합. Western blot 및 면역세포염색에 사용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체. 액상 형태로 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human beta actin. This sequence is identical to similar regions in all four alpha actins, as well as in gamma actin, and is well conserved in actins from most eukaryotic species. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942871 |
Product Specific Information
This antibody detects a 42 kDa protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate-treated human C2C12 and SYF cSrc transformed cells, but not in control cells.
In addition, this antibody either detects actin cleavage products at 36 and 20 kDa or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.
Target Information
Actin is a ubiquitous protein involved in filament formation that constitutes a large portion of the cytoskeleton.
Actin filaments interact with myosin to assist in muscle contraction, cell motility, and cytokinesis.
In vertebrates, there are three groups of actin isoforms: alpha, beta, and gamma.
- Alpha actins: Found in muscle tissues; major constituents of the contractile apparatus.
- Beta and gamma actins: Co-exist in most cell types as cytoskeletal components and mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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