Thermo Fisher Scientific CD130 (GP130) Monoclonal Antibody (KGP130), APC, eBioscience
마우스 gp130(CD130)를 인식하는 단클론 항체로, APC 형광체가 결합되어 있습니다. Flow cytometry용으로 검증되었으며, IL-6 수용체 신호전달 연구에 적합합니다. 0.25 µg/test 권장, 4°C 암소 보관.
- 카탈로그번호
- 17-1302-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD130 (GP130) Monoclonal Antibody (KGP130), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 4 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KGP130 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10671133 |
Additional Formats
Product Specific Information
The KGP130 monoclonal antibody reacts with mouse gp130 (CD130), a 130 kD type I transmembrane glycoprotein with a 597 amino acid extracellular domain, a single transmembrane domain, and a 277 amino acid cytoplasmic domain. gp130 is a subunit of several heterodimeric cell-surface receptors, including IL-6, IL-11, IL-27, Oncostatin M, and LIF receptors. A soluble form of gp130 can inhibit IL-6 signaling.
gp130 is mainly expressed on T cells, monocytes, endothelial cells, activated B cells, and plasma cells, with lower expression on most leukocytes and epithelial cells. Upon ligand binding, gp130 becomes tyrosine phosphorylated, activating signaling pathways such as PI3 kinase, Ras-MAPK, and STAT.
Preincubation of mouse splenic T cells with IL-6 can block KGP130 binding, suggesting the KGP130 epitope overlaps the IL-6 interaction site. No reactivity with human gp130 has been observed.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test.
A test is defined as the amount of antibody staining a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).
Optimize dilution for best performance.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The gene encodes a signal transducer shared by multiple cytokines, including IL-6, CNTF, LIF, and OSM. It functions as part of the cytokine receptor complex, activated upon cytokine binding. The viral IL-6 (vIL6) protein from Kaposi sarcoma-associated herpesvirus can directly activate this protein. Mouse knockout studies indicate a role in regulating myocyte apoptosis. Alternative splicing produces distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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