Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (YB5.B8), APC, eBioscience
인간 CD117(c-Kit)을 인식하는 YB5.B8 단클론 항체로, APC 형광표지되어 유세포분석(Flow Cytometry)에 최적화됨. 조혈 전구세포 및 비만세포 표지에 사용. 633–647 nm 레이저에서 660 nm 방출. 액상 형태로 4°C 암소 보관.
- 카탈로그번호
- 17-1179-42
- 판매단위
- pk
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Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (YB5.B8), APC, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 7 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | YB5.B8 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10596820 |
Additional Formats
Product Specific Information
The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit, Steel factor receptor and stem cell factor receptor. This 145 kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cells and mast cells. Interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in their activation.
For staining HSCs from peripheral blood, clone 104D2 is recommended. Refer to Product #17-1178.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on TF-1 (human erythroleukemia) cell line at 5 µL (0.5 µg)/test.
A test is defined as the amount of antibody staining a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, stem cell factor). KIT regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of KIT receptor TK (e.g., mutation D816V) is a therapeutic target in neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract cajal cells. Upon SCF binding, c-Kit dimerizes, leading to receptor activation and autophosphorylation, enabling downstream signaling pathways (Ras/ERK, PI3K, PLC-γ, JAK/STAT). c-Kit activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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