Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), APC, eBioscience
마우스 CD117(c-Kit) 인식 단클론 항체로, APC 형광 표지되어 있으며 유세포분석에 적합합니다. 조혈모세포, 비만세포 표지에 사용되며 높은 특이도와 재현성을 제공합니다. 4°C 암소 보관, 동결 금지.
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- 17-1171-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 3 |
| Flow Cytometry (Flow) | 0.125 µg/test | 164 |
| Functional Assay | - | 1 |
| Affinity Purification (AP) | - | 1 |
| Miscellaneous (PubMed) | - | 5 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Pig |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B8 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469429 |
Additional Formats Available
- Unconjugated (14-1171-82)
- APC-eFluor 780 (47-1171-82)
- Biotin (13-1171-82)
- Brilliant UV 395 (363-1171-82)
- Brilliant UV 563 (365-1171-82)
- Brilliant UV 615 (366-1171-82)
- Brilliant UV 661 (376-1171-82)
- Brilliant UV 737 (367-1171-82)
- Brilliant Violet 421 (404-1171-82)
- Brilliant Violet 480 (414-1171-82)
- Brilliant Violet 605 (406-1171-82)
- Brilliant Violet 650 (416-1171-82)
- Brilliant Violet 786 (417-1171-82)
- eFluor 450 (48-1171-82)
- FITC (11-1171-82)
- PE (12-1171-82)
- PE-Cyanine5 (15-1171-82)
- PE-Cyanine7 (25-1171-82)
- PE-eFluor 610 (61-1171-82)
- PerCP-eFluor 710 (46-1171-82)
- Super Bright 436 (62-1171-82)
- Super Bright 600 (63-1171-82)
- Super Bright 645 (64-1171-82)
- Super Bright 702 (67-1171-82)
- Super Bright 780 (78-1171-82)
- Request custom conjugation available
Product Specific Information
The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed on most hematopoietic progenitor cells in mouse bone marrow, as well as on mast cells. The c-Kit and steel factor interaction promotes proliferation and differentiation of hematopoietic progenitor cells and is involved in mast cell activation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of mouse bone marrow cells at ≤0.125 µg/test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume. Cell number may vary (10⁵–10⁸ cells/test). Titrate for optimal performance.
Fluorophore properties:
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for mast cell growth factor (stem cell factor). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, AML, and piebaldism. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and interstitial cells of Cajal. Upon ligand binding, c-Kit dimerizes and autophosphorylates, activating downstream pathways such as Ras/ERK, PI3K, PLC-γ, and JAK/STAT, essential for hematopoiesis and stem cell maintenance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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