Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated (14-1171-82)
• APC-eFluor 780 (47-1171-82)
• Biotin (13-1171-82)
• Brilliant UV 395 (363-1171-82)
• Brilliant UV 563 (365-1171-82)
• Brilliant UV 615 (366-1171-82)
• Brilliant UV 661 (376-1171-82)
• Brilliant UV 737 (367-1171-82)
• Brilliant Violet 421 (404-1171-82)
• Brilliant Violet 480 (414-1171-82)
• Brilliant Violet 605 (406-1171-82)
• Brilliant Violet 650 (416-1171-82)
• Brilliant Violet 786 (417-1171-82)
• eFluor 450 (48-1171-82)
• FITC (11-1171-82)
• PE (12-1171-82)
• PE-Cyanine5 (15-1171-82)
• PE-Cyanine7 (25-1171-82)
• PE-eFluor 610 (61-1171-82)
• PerCP-eFluor 710 (46-1171-82)
• Super Bright 436 (62-1171-82)
• Super Bright 600 (63-1171-82)
• Super Bright 645 (64-1171-82)
• Super Bright 702 (67-1171-82)
• Super Bright 780 (78-1171-82)
• Request custom conjugation available

Product Specific Information

The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed on most hematopoietic progenitor cells in mouse bone marrow, as well as on mast cells. The c-Kit and steel factor interaction promotes proliferation and differentiation of hematopoietic progenitor cells and is involved in mast cell activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of mouse bone marrow cells at ≤0.125 µg/test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume. Cell number may vary (10⁵–10⁸ cells/test). Titrate for optimal performance.

Fluorophore properties:

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for mast cell growth factor (stem cell factor). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, AML, and piebaldism. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and interstitial cells of Cajal. Upon ligand binding, c-Kit dimerizes and autophosphorylates, activating downstream pathways such as Ras/ERK, PI3K, PLC-γ, and JAK/STAT, essential for hematopoiesis and stem cell maintenance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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