Thermo Fisher Scientific IL-1 beta Polyclonal Antibody, HRP

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as undiluted liquid; dilute only before immediate use.
• Heated to 56°C for 30 minutes.
• IgG preparation of whole rabbit serum purified by DEAE fractionation.
• Directed against mature 17 kDa mouse IL-1β; does not recognize human IL-1β or mouse IL-1α.
• Reacts with rat IL-1β in ELISA and other immunoassays.
• Recognizes 10% of non-denatured 31 kDa precursor IL-1β; primarily detects mature 17 kDa form.
• Denatured 31 kDa precursor IL-1β recognized in immunoblot analysis.

Target Information

Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized as a 31 kDa inactive pro-form that accumulates in the cytosol and is cleaved into an active 17 kDa form upon inflammasome activation. Caspase-1 activation mediates this cleavage. IL-1β lacks a signal peptide for the classical ER/Golgi pathway and is secreted via an alternate mechanism. It signals through IL-1RI and IL-1RII receptors, shared with IL-1α, and its activity is regulated by IL-1 Receptor Antagonist (IL-1RA). IL-1β plays a key role in innate immunity and inflammation; elevated levels are associated with chronic inflammatory diseases, making IL-1β neutralizing antibodies of potential therapeutic interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.