Thermo Fisher Scientific IL-1 beta Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IL-1 beta Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-1β. Anti-Murine IL-1β-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mIL-1β by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-1β (500-P51) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant mIL-1β.

Western Blot:
To detect mIL-1β by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant mIL-1β is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package:
500-P51BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized as a 31 kDa inactive pro-form that is cleaved into a 17 kDa active form via inflammasome activation. IL-1β signals through IL-1RI and IL-1RII receptors, shared with IL-1α, and its activity is regulated by IL-1 Receptor Antagonist (IL-1RA). IL-1β plays a key role in innate immune responses and is associated with various chronic inflammatory conditions.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.