Thermo Fisher Scientific IL-1 beta Polyclonal Antibody, PeproTech
Murine IL-1β를 검출하기 위한 Rabbit Polyclonal Antibody. Western blot, ELISA, IHC 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체로 높은 특이성과 감도를 제공. 연구용으로만 사용 가능.
- 카탈로그번호
- 500-P51-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-1 beta Polyclonal Antibody, PeproTech
Thermo Fisher Scientific IL-1 beta Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | 3 publications |
| Immunohistochemistry (IHC) | – | 1 publication |
| ELISA | 0.5–2.0 µg/mL | 3 publications |
| Blocking Assay (BLOCK) | – | 1 publication |
| In vitro Assay (IV) | – | 2 publications |
| Immunostaining (IS) | – | 4 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Bacteria, Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Recombinant Murine IL-1β |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930599 |
Product Specific Information
AA Sequence of Recombinant Protein:
MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-1β. Anti-Murine IL-1β-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-1β matrix.
Sandwich ELISA:
To detect Murine IL-1β by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. When used with PeproTech Biotinylated Anti-Murine IL-1β (500-P51Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-1β.
Western Blot:
To detect Murine IL-1β by Western Blot, use at 0.1–0.2 µg/mL. Detection limit for Recombinant Murine IL-1β is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized as a 31 kDa inactive pro-form that requires inflammasome activation for cleavage into the active 17 kDa form via caspase-1 processing.
IL-1β lacks a signal peptide and is secreted through a non-classical pathway. It signals through IL-1RI and IL-1RII receptors, shared with IL-1α. Its activity is regulated by IL-1 Receptor Antagonist (IL-1RA).
IL-1β plays a crucial role in innate immune defense and is associated with chronic inflammatory conditions. Neutralizing antibodies against IL-1β hold therapeutic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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