Thermo Fisher Scientific IL-1 alpha Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IL-1 alpha Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-1alpha. Anti-Murine IL-1alpha-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mIL-1alpha by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-1alpha (500-P51A) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant mIL-1alpha.

Western Blot:
To detect mIL-1alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant mIL-1alpha is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P51ABT-1MG will be provided as 2 × 500 µg.

Target Information

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is encoded by the IL1A gene and signals through IL-1RI and IL-1RII receptors, shared with IL-1 beta. Located on chromosome 2q13, IL-1 alpha plays a key role in innate host defense by inducing other proinflammatory cytokines and acute-phase responses.
Its activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), which inhibits receptor binding competitively. IL-1 alpha participates in immune responses, inflammation, and hematopoiesis. It is produced as a proprotein by monocytes and macrophages, processed and released upon cell injury, and induces apoptosis.
The IL1A gene is part of a cytokine gene cluster on chromosome 2 along with eight other IL-1 family genes. IL-1 alpha regulates NF-kappaB and MAPK pathways, stimulates IL6 and COX-2 expression, and enhances nitric oxide production. Elevated IL-1 alpha levels are linked to chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.