Thermo Fisher Scientific Catenin beta (CTNNB1) Recombinant Rabbit Monoclonal Antibody (CTNNB1/7044R)

Applications

• Immunohistochemistry (Paraffin) (IHC-P)

Tested Dilution

• 1–2 µg/mL

Product Specifications

Product Specific Information

• Antibody is stable for 24 months.
• Positive Control: Human breast carcinoma
• Cellular Localization: Cell surface, cytoplasm

Specificity Comments:
Beta-catenin associates with the cytoplasmic portion of E-cadherin, essential for its adhesion function. In normal tissues, beta-catenin localizes to epithelial cell membranes. In breast ductal neoplasia, it is membrane-localized, while in lobular neoplasia, it redistributes throughout the cytoplasm, showing diffuse staining. Immunostaining of beta-catenin and E-cadherin aids in distinguishing ductal and lobular neoplasms, including DCIS and lobular carcinoma. Some rectal and gastric adenocarcinomas exhibit diffuse cytoplasmic beta-catenin staining, similar to lobular breast carcinomas.

Target Information

Beta-catenin is an adherens junction (AJ) protein involved in cell-cell adhesion and epithelial layer maintenance. It interacts with E-cadherin and alpha-catenin, linking to the cortical actin cytoskeleton. Beta-catenin also binds to complexes containing adenomatous polyposis coli protein and microtubules, engaging signaling pathways such as Wnt/Wingless. It plays roles in morphogenesis and is expressed in various hair follicle cell types. Pathological roles have been observed in pilomatrixoma, medulloblastoma, colorectal, ovarian, and other cancers. In the nucleus, beta-catenin coactivates Lef/Tcf transcription factors, promoting expression of cyclin D and c-myc, key regulators of cell proliferation and tumorigenesis.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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