Thermo Fisher Scientific Phospho-Histone H3 (Thr11) Recombinant Rabbit Monoclonal Antibody (RM164)
Histone H3 Thr11 인산화 형태를 특이적으로 인식하는 재조합 토끼 단클론 항체. Western blot, ELISA, Luminex 등에 적합. 로트 간 일관성, 높은 특이도와 감도를 제공하며, 동물 유래 성분이 없는 포뮬레이션.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex (LUM) | 0.1–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM164 |
| Immunogen | Phospho-peptide corresponding to Phospho-Histone H3 (Thr11) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661904 |
Product Specific Information
This antibody reacts specifically with Histone H3 phosphorylated at Threonine 11, showing no cross-reactivity with other phosphorylated histones.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histones, it forms nucleosomes that package DNA. Histones are central to transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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