Thermo Fisher Scientific Phospho-Histone H3 (Thr3) Recombinant Rabbit Monoclonal Antibody (RM159)
Histone H3의 Threonine 3 인산화 형태를 특이적으로 인식하는 Recombinant Rabbit Monoclonal 항체. Western blot, IHC, ICC, ELISA 등에 사용 가능. 높은 특이성과 재현성, 동물 유래 성분 없음. Thermo Fisher Scientific 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (IHC) | 0.5–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex (LUM) | 0.1–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM159 |
| Immunogen | Phospho-peptide corresponding to Phospho-Histone H3 (Thr3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661907 |
Product Specific Information
This antibody reacts specifically with Histone H3 phosphorylated at Threonine 3, showing no cross-reactivity with other phosphorylated histones.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histones, it forms the nucleosome structure that organizes DNA. Histones are central to transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes various post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and transcription factor binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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