Thermo Fisher Scientific H3K79me1 Recombinant Rabbit Monoclonal Antibody (RM147)
Histone H3의 Lys79 단일메틸화(K79me1)에 특이적인 재조합 토끼 단클론 항체. Western blot, ELISA, ChIP, Luminex에 사용 가능. 높은 특이성과 로트 간 일관성, 동물 유래 성분 없음. -20°C 보관, 단백질 A 정제, PBS/glycerol buffer.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–1 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 2–10 µg/mL |
| Luminex (LUM) | 0.1–0.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM147 |
| Immunogen | Monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys79) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661908 |
Product Specific Information
This antibody reacts specifically to Histone H3 monomethylated at Lysine 79 (K79me1).
No cross-reactivity with dimethylated (K79me2), trimethylated (K79me3), or other methylations in histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it forms the nucleosome structure.
Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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