Thermo Fisher Scientific H3K18me1 Recombinant Rabbit Monoclonal Antibody (RM167)
Histone H3K18me1에 특이적인 재조합 토끼 단클론 항체로 높은 특이성과 민감도 제공. Western blot, ELISA, Luminex 등 다양한 응용 가능. 로트 간 일관성 우수하며 동물 유래 성분이 없는 포뮬레이션. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex | 0.1–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM167 |
| Immunogen | Monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys18) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661917 |
Product Specific Information
This antibody reacts specifically to Histone H3 monomethylated at Lysine 18 (K18me1).
No cross-reactivity with dimethylated (K18me2), trimethylated (K18me3), or other methylations in histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems.
These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening and selection of optimal clones for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Histone H3 is one of the DNA-binding proteins found in eukaryotic chromatin.
Together with four core histone proteins, it forms the nucleosome structure.
Histones are central to transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo various post-translational modifications such as:
- Methylation or acetylation of lysine and arginine residues
- Phosphorylation of serine or threonine residues
These modifications influence chromatin structure and gene expression by enabling binding of specific transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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