Thermo Fisher Scientific Phospho-Histone H3 (Thr6) Recombinant Rabbit Monoclonal Antibody (RM160)
Histone H3의 Thr6 위치 인산화 형태를 특이적으로 인식하는 recombinant rabbit monoclonal antibody입니다. Western blot, ELISA, Luminex 등 다양한 응용에 적합하며, 높은 특이성과 재현성을 제공합니다. 동물 유래 성분이 없는 in vitro 시스템에서 생산되어 로트 간 일관성이 우수합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex (LUM) | 0.1–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM160 |
| Immunogen | Phospho-peptide corresponding to Phospho-Histone H3 (Thr6) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661903 |
Product Specific Information
This antibody reacts specifically to Histone H3 phosphorylated at Threonine 6, showing no cross-reactivity with other phosphorylated histones.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in eukaryotic chromatin. Together with other core histones, it forms nucleosomes that play key roles in:
- Transcription regulation
- DNA repair
- DNA replication
- Chromosomal stability
The N-terminal tail of histone H3 undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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