Thermo Fisher Scientific ERK2 Internal Monoclonal Antibody (3H9.A1.A8), DyLight 800

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

ELISA (ELISA)

1:40,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

3H9.A1.A8

Immunogen

Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.

Conjugate

DyLight™ 800 DyLight™ 800 DyLight™ 800

View additional formats

• Unconjugated
• Biotin
• DyLight 680
• FITC
• HRP
• Request custom conjugation

Excitation/Emission Max

789/794 nm View spectra

Form

Lyophilized

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reacts specifically with human ERK2. Anti-ERK2 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK1 isoform.

Target Information

MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.