Thermo Fisher Scientific MEK2 C-Term Monoclonal Antibody (12A6.G1.G11), DyLight 800
MEK2 단백질의 C-말단을 인식하는 DyLight 800 결합 단일클론 항체로, Western blot 및 ELISA에 적합합니다. 인간, 생쥐, 랫트에 반응하며, 높은 특이성과 재현성을 제공합니다. 단백질 A 정제 및 안정한 동결건조 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12A6.G1.G11 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the C-terminus of MEK2 conjugated to KLH |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats Available
- Unconjugated
- Biotin
- DyLight 680
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid repeated freeze/thaw cycles. Centrifuge if solution is not clear after standing. Stable for several weeks at 4°C as undiluted liquid. Dilute only immediately before use.
This antibody reacts specifically with human MEK2 and is expected to cross-react with mouse and rat MEK2 based on sequence identity. It does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction. It activates ERK1/2 and MAPK enzymes by phosphorylating both residues within the TEY motif in the activation loop. MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222. MEK1 is further regulated by phosphorylation at threonine 292 by ERK2, which provides negative feedback regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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