Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), eFluor 660, eBioscience
Mouse IL-13을 인식하는 단일클론 항체로, eFluor 660 형광체가 결합되어 Flow Cytometry에 적합합니다. Th2 세포 유래 IL-13 검출에 사용되며, 0.25 µg/test로 최적화된 성능을 제공합니다. 알레르기 및 면역반응 연구용으로 활용됩니다.
- 카탈로그번호
- 50-7133-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), eFluor 660, eBioscience
Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 41 publications |
| ELISA | - | View 1 publication |
| Neutralization | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio13A |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574278 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC-eFluor 780
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBio13A antibody reacts with mouse IL-13, a cytokine produced mainly by Th2 cells and antigen-primed CD8 T cells. IL-13 is involved in allergic inflammation, parasitic clearance, and cancer models where it may have inhibitory or stimulatory effects depending on the tumor type. In humans, IL-13 plays a role in B cell isotype switching and modulates macrophage activity by reducing pro-inflammatory cytokines such as IL-1, IL-6, IL-8, IL-10, and IL-12.
Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on Th2 polarized mouse splenocytes; optimal performance at ≤0.25 µg/test. A test is defined as staining a cell sample in a final volume of 100 µL, with cell numbers ranging from 10⁵–10⁸ cells/test. Antibody titration is recommended for assay optimization.
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, and is excited with the red laser (633 nm). Ensure instrument compatibility.
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It regulates inflammatory and immune responses by down-regulating macrophage activity and inhibiting pro-inflammatory cytokine production. IL-13 is implicated in allergen-induced asthma through IgE-independent mechanisms and influences B cell maturation, differentiation, and IgE isotype switching. Structurally, IL-13 resembles IL-3, IL-4, IL-5, and GM-CSF. The IL-13 gene is located on chromosome 5q within a cytokine gene cluster near IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.