Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), eFluor 660, eBioscience
Mouse 및 Rat IL-17A를 특이적으로 인식하는 단클론 항체로, eFluor 660 형광표지로 Flow Cytometry에 최적화됨. 염증성 사이토카인 연구 및 Th17 세포 분석에 적합. 0.25 µg/test로 사용 가능하며, 4°C 암소 보관.
- 카탈로그번호
- 50-7177-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 5 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | 1 |
| Flow Cytometry (Flow) | 0.25 µg/test | 85 |
| ELISA | - | 3 |
| Functional Assay | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio17B7 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651/668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_11218697 |
Additional Formats Available
- Alexa Fluor 488
- APC
- Biotin
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- eFluor 506
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
Description:
The eBio17B7 antibody reacts with mouse and rat IL-17A without recognizing IL-17F. IL-17A is a CD4+ T cell-derived cytokine promoting inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. It induces expression of ICAM-1 and secretion of IL-6, IL-8, G-CSF, and PGE2 in various cell types, and supports hematopoietic progenitor proliferation and neutrophil maturation.
Applications Reported:
Reported for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of restimulated, Th17-polarized mouse splenocytes using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00).
Recommended usage ≤ 0.25 µg/test in 100 µL final volume. Cell number range: 10⁵–10⁸ cells/test.
Staining also compatible with the Foxp3 buffer system (Product # 00-5523-00).
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, excited by 633 nm red laser.
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red.
Filtered through 0.2 µm post-manufacturing.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses. It is elevated in autoimmune and inflammatory diseases such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A forms a 32 kDa disulfide-linked homodimer and signals through IL-17R/CDw217. It regulates NF-kappa B and MAPK pathways, stimulating IL-6 and COX-2 expression and enhancing nitric oxide production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.