Thermo Fisher Scientific IL-17F Monoclonal Antibody (SHLR17), eFluor 660, eBioscience

Thermo Fisher Scientific IL-17F Monoclonal Antibody (SHLR17), eFluor 660, eBioscience

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution: 5 µL (0.125 µg)/test
Applications Reported: Intracellular staining followed by flow cytometric analysis
Applications Tested: Pre-titrated and validated for Th17-polarized peripheral blood cells

Product Specifications

Additional Formats Available

• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 650
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

The SHLR17 monoclonal antibody reacts with human interleukin (IL)-17F, a 34 kDa homodimeric cytokine belonging to the IL-17 family. IL-17F and IL-17A are produced primarily by Th17 cells and exhibit overlapping yet distinct functions. IL-17F binds to a heterodimeric receptor complex (IL-17RA/IL-17RC), inducing secretion of pro-inflammatory cytokines and chemokines, and promoting neutrophil recruitment.

This antibody is validated for intracellular staining followed by flow cytometric analysis. Recommended usage is 5 µL (0.125 µg) per test in a final volume of 100 µL. Cell number can range from 10⁵ to 10⁸ cells/test depending on experimental conditions.

eFluor® 660 serves as a replacement for Alexa Fluor® 647, excited by a red laser (633 nm) and emitting at 659 nm. Ensure instrument compatibility with this fluorochrome.

Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

IL-17F (Interleukin 17F, CTLA-8) is a cytokine produced by activated T cells, mast cells, and basophils. It plays a key role in allergic airway inflammation and induces cytokines and chemokines in bronchial epithelial cells, endothelial cells, fibroblasts, and eosinophils. IL-17F may form homodimers or heterodimers with IL-17A, binding to IL-17R to recruit monocytes and neutrophils and stimulate production of TNF-α, IL-1β, IL-6, and IL-8. IL-17F is implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지