Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), eFluor 660, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 8 publications 8 publications

ELISA (ELISA)

-

View 4 publications 4 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES3-9D7

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

View additional formats

• Unconjugated
• Alexa Fluor 488
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149363

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: This JES3-9D7 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining anf flow cytometryci analysis of Th2 polarized and stimulated human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.