Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), eFluor 660, eBioscience
인간 IL-10을 인식하는 JES3-9D7 단클론 항체로, eFluor 660 형광 표지. Flow cytometry 및 intracellular staining에 최적화. 5 µL/test로 사용, 633 nm 레이저에 적합. 연구용으로 면역조절 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 2 |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | 8 |
| ELISA | - | 4 |
| Functional Assay | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor 660, eBioscience |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES3-9D7 |
| Conjugate | eFluor 660 |
| Excitation/Emission Max | 651/668 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11149363 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Applications reported include flow cytometric analysis and intracellular staining.
This antibody has been pre-titrated and tested by intracellular staining and flow cytometry analysis of Th2-polarized and stimulated human peripheral blood cells.
Recommended use: 5 µL (0.125 µg) per test. A test is defined as the amount of antibody sufficient for staining a sample in a final volume of 100 µL. Cell number can range from 10⁵ to 10⁸ cells/test.
eFluor 660 is a replacement for Alexa Fluor 647, emits at 659 nm, and is excited with the red laser (633 nm). Ensure your instrument supports this fluorochrome.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
IL-10 encodes a cytokine primarily produced by monocytes and some lymphocytes. It plays a key role in immunoregulation and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It promotes B cell survival, proliferation, and antibody production, and can inhibit NF-kappa B activity. IL-10 is also involved in the JAK-STAT signaling pathway. Knockout studies in mice indicate its importance in intestinal immune regulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and HIV Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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