Thermo Fisher Scientific Zap-70 Polyclonal Antibody
ZAP-70 단백질을 인식하는 Rabbit Polyclonal Antibody로, 인간 및 마우스에 반응합니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며 장기 보관은 -20°C에서 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ZAP70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792269 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (95%), Rat (92%), Bovine (93%).
- Positive Control: Jurkat cells.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region between SH2 and kinase domains.
Phosphorylation at tyrosine 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while phosphorylation at tyrosine 292 attenuates activation.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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