Thermo Fisher Scientific Phospho-c-Raf (Ser642) Polyclonal Antibody
Human c-Raf 단백질의 Ser642 인산화 부위를 인식하는 rabbit polyclonal antibody. Western blot에 적합하며 HUVEC 또는 K562 세포를 양성 대조군으로 권장. 액상 형태, 1 mg/mL 농도, -20°C 보관. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
- Suggested Positive Control: HUVEC or K562 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of Serine 642 (T-T-S(p)-P-R) derived from Human C-RAF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554319 |
Product Specific Information
A suggested positive control for Western blot is HUVEC or K562 cells.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) that functions downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates the dual specificity protein kinases MEK1 and MEK2, which in turn activate ERK1 and ERK2. Activated ERKs regulate gene expression related to cell division, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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