Thermo Fisher Scientific Phospho-c-Raf (Thr269) Polyclonal Antibody
Phospho-c-Raf (Thr269) 폴리클로날 항체로 인간 및 생쥐 시료에 반응합니다. IHC 및 ICC/IF 실험에 적합하며, 고순도 친화 크로마토그래피 정제 제품입니다. 액상 형태로 -20°C 보관, 연구용으로만 사용됩니다.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:100
Immunocytochemistry (ICC/IF)
- Tested Dilution: 5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of threonine269 (S-T-T(p)-L-P) derived from Human C-RAF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554320 |
Product Specific Information
- A suggested positive control for IHC is human brain tissue.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which subsequently activate ERK1 and ERK2. Activated ERKs regulate gene expression involved in cell division, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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