Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), APC, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 0.25 µg/test
• Publications: View 2 publications

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4 beta1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), which are important for early lymphocyte and myeloid development in bone marrow and thymus. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role in inflammatory responses.

Applications Reported:
This APC antibody has been reported for use in flow cytometric analysis.

Applications Tested:
This APC antibody has been tested by flow cytometric analysis of mouse bone marrow cells. It may be used at ≤0.25 µg per test (defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Excitation / Emission / Laser:
633–647 nm / 660 nm / Red Laser

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It plays roles in leukocyte migration, leukocyte-endothelial adhesion, and signal transduction, and may be involved in inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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