Thermo Fisher Scientific CD101 Monoclonal Antibody (Moushi101), APC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 2 publications

Product Specifications

Additional Formats

• Alexa Fluor 700
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The monoclonal antibody Moushi101 recognizes mouse CD101, also known as IGSF2 and V7 (7 IgV domains).
CD101 is a transmembrane glycoprotein containing seven Ig-like domains and a short cytoplasmic tail with phosphorylation sites. Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation.

CD101 is expressed on:

• Monocytes
• Granulocytes
• Dendritic cells
• Langerhans cells
• Activated T cells

It is also found on a subset of CD25⁺Foxp3⁺ regulatory T cells with high suppressive properties in vitro and in vivo.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse bone marrow cells.
Recommended use ≤ 0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Careful titration is recommended for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser

Target Information

CD101 (V7 antigen) is a member of the immunoglobulin superfamily and forms a disulfide-linked homodimer. It is expressed on monocytes, granulocytes, dendritic cells, and certain T lymphocytes. CD101⁺CD28⁺ cells respond strongly to CD28 signaling, and co-stimulation with anti-CD28 or suboptimal anti-CD3 can enhance proliferation, suggesting an activating role.

In mice, CD101 is found on a subpopulation of CD4⁺CD25⁺Foxp3⁺ regulatory T cells with high suppressor activity. In human PBMCs, CD101 expression is observed in ~30% of Foxp3⁺ cells, though enhanced suppressor activity has not been confirmed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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